Improved techniques for collecting motile spermatozoa from human semen.
نویسندگان
چکیده
منابع مشابه
Sperm tail flexibility test: a simple test for selecting viable spermatozoa for intracytoplasmic sperm injection from semen samples without motile spermatozoa.
PURPOSE The objective was to describe the results of the injection of immotile spermatozoa with flexible tails when only immotile spermatozoa are present in the semen sample. METHODS A retrospective study was conducted to analyze the procedure results for 10 couples who participated in our intracytoplasmic sperm injection program. The sperm tail was considered flexible when it moved up and do...
متن کاملA method of concentrating spermatozoa in human semen.
some hours must elapse between ejaculation and insemination the viability of the sperm may be improved by the addition of suitable diluents, as is the usual practice in animal insemination. In human semen, however, the improved motility due to dilution is seldom enough to compensate for the reduction in density, unless the sperm are subsequently concentrated by centrifuging or some other means....
متن کاملProcessing of semen by density gradient centrifugation selects spermatozoa with longer telomeres for assisted reproduction techniques.
The ends of eukaryotic chromosomes contain specialized chromatin structures called telomeres, the length of which plays a key role in early human embryonic development. Although the effect of sperm preparation techniques on major sperm characteristics, such as concentration, motility and morphology have been previously documented, the possible status of telomere length and its relation with spe...
متن کاملTechniques for augmentation of exogenous DNA uptake by ovine spermatozoa
Sperm mediated gene transfer can be an inexpensive and simple method in animal transgenesis; however its efficiency is poor, mainly due to the spermatozoa’s lesser uptake of exogenous DNA. In the present study, the effects of lipofection and other augmentation techniques, such as sperm freezing and spermatozoa treatment with triton X100 and DMSO, on exogenous DNA uptake by sheep spermatozoa and...
متن کاملAgglutination of Human Spermatozoa Due to Human Semen Culture Bacterial Isolates Bearing Sperm Ligand
Sperm ligand on Escherichia coli that agglutinates human spermatozoa has been extracted from washed E. coli cells by incubating with 3M NaCl for 12 h at 37oC under shake conditions (150 rpm). Further the sperm ligand was purified after extensive dialysis against double distilled water by step down chromatography procedures (molecular weight sieving, anion exchange). The purification procedure y...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: International Journal of Andrology
سال: 1984
ISSN: 0105-6263,1365-2605
DOI: 10.1111/j.1365-2605.1984.tb00760.x